Table 1.
Properties of the Hbl complexes excised from Blue Native gels. WB: detection of Hbl components in Western blots; LC-MS: detection of Hbl components via additionally performed liquid chromatography mass spectrometry; −: no signal; (+): weak signal; +: clear signal; ++: strong signal; +++: very strong signal; nd: not determined.
| Band No | Approximate Size (kDa) | Hbl B WB | Hbl L1 WB | Hbl L2 WB | Pore Formation | Additional LC-MS |
|---|---|---|---|---|---|---|
| B. cereus supernatant | ||||||
| 1 | 700 | (+) | − | − | + | + |
| 2 | 600 | + | − | − | + | + |
| 3 | 440 | + | + | − | − | + |
| 4 | 300 | + | + | − | − | nd |
| 5 | 200 | + | + | + | − | nd |
| 6 | 140 | + | + | + | − | nd |
| 7 | 120 | + | + | + | − | + |
| 8 | 100 | + | + | + | ++ | nd |
| 9 | 70 | + | + | + | − | + |
| rHbl | ||||||
| 10 | 440 | + | + | + | ++ | + |
| 11 | 300 | + | + | + | + | + |
| 12 | 200 | + | + | + | ++ | + |
| 13 | 160 | + | + | + | +++ | nd |
| 14 | 140 | + | + | + | +++ | nd |
| 15 | 120 | + | + | + | + | nd |