Figure 5.
Effect of NAC on iron-induced migration in human endometriotic cells. Human endometriotic 12Z cells were treated with iron (FeSO4: 100 µM; FAC: 100 µg/mL) in the presence of 5 mM of N-acetyl-l-cysteine (NAC) for 24 h. (A) Transwell migration assay was performed. (B) The protein expressions of MMP-2 and MMP-9 were determined by Western blot analysis. β-Actin was used as a loading control. Representative images of three independent experiments are shown. The values represent the mean ± SD of results from three independent experiments. * p < 0.05 as compared the control group; # p < 0.05 as compared the treated group.