Figure 2.

WFA specifically recognize non-sulfated tetrasaccharides (A), Biotinylated Chn and GAG polymers (CS-A, CS-B, CS-C, CS-D, and heparin) (a) and chemically synthesized biotinylated tetrasaccharides (O-O, A-A, C-C, D-D, and E-E) (b) were immobilized onto a streptavidin-coated plate, then horseradish-conjugated WFA was added to each well. WFA binding to immobilized GAGs or tetrasaccharides was measured by ELISA. (B) WFA binding to O-O tetrasaccharides digested with or without β-HexNAcase was examined.