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. 2020 Oct 30;10(11):1499. doi: 10.3390/biom10111499

Figure 3.

Figure 3

The Sema3A binding motif is partially recognized by Cat316 and WFA. (A) Binding of Sema3A to biotinylated Chn and GAG polymers (CS-A, CS-B, CS-C, CS-D, and heparin) was examined by ELISA. (B) Binding of Sema3A to biotinylated tetrasaccharides, O-O, was examined by ELISA. (C) Binding of Sema3A to A-A, C-C, D-D, and E-E tetrasaccharides is shown as a percentage of the binding of Sema3A to O-O tetrasaccharides. (D) Binding of Sema3A and Cat316 to O-O tetrasaccharides was examined after O-O tetrasaccharides were digested with β-HexNAcase for 0, 4, and 24 h. (E) Binding of Cat316 to O-O tetrasaccharides was examined after O-O tetrasaccharides were incubated with Sema3A (0, 400, 800, and 1600 ng/mL). (F) Binding of WFA to O-O tetrasaccharides was inhibited using Sema3A (0, 400, 800, and 1600 ng/mL) as a competitor. (G) The effect of Cat316 pretreatment on the binding of Cat316 (a) and Sema3A (b).