Table 1.
HA or HEP endocytosis utilizes multiple HARE CD motifs. Details of the two studies are in Pandey et al. [58,59]. The organization and sequences of the four CD motifs targeting HARE to clathrin-coated pits (M1–M4) are shown in Figure 5. Results in boldface (ΔM2 and ΔM4) indicate a motif that mediates endocytosis of either one of, but not both, HA and Hep.
| HARE VARIANT | HA UPTAKE (%) | HEP UPTAKE (%) |
|---|---|---|
| WT | 100 | 100 |
| EV | 0.2 | 0 |
| ΔM1 | 51 | 65 |
| ΔM2 | 61 | 135 |
| ΔM3 | 44 | 35 |
| ΔM4 | 119 | 68 |
| ΔM1M2 | 61 | - |
| ΔM1M2M4 | 58 | - |
| ΔM1M2M3M4 | 7 | - |
| WT(Y2519A) | 94 | 95 |
| ΔM1M2M3 + Y2519A | 5 | 0 |
| +M2 or +M4 | 0 | 0-5 |
| +M3 | 58 | 65 |
| +M3(Y2519A) | 5 | 0 |