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. 2020 Nov 4;9(11):1083. doi: 10.3390/antiox9111083

Figure 2.

Figure 2

Thymus vulgaris essential oil (TEO: 25, 150, and 300 μL/L) improved locomotion pattern and reduced anxiety in the novel tank diving (NTT) test. (A) Representative locomotion tracking pattern of the control, scopolamine (Sco: 100 μM), and TEO (25, 150, and 300 μL/L) treated groups. (B) Represent the time spent in the top/bottom zone by zebrafish in the tank in different groups. (C) Represent the total distance travelled by zebrafish in the tank in different groups. (D) Represent the average velocity of the zebrafish in different groups. Values are means ± S.E.M. (n = 10). For Tukey’s post hoc analyses: (B) Control vs. Sco (100 μM): ### p < 0.0001, Sco (100 μM) vs. TEO (25 μL/L): ### p < 0.0001, Sco (100 μM) vs. TEO (150 μL/L): ### p < 0.0001, and Sco (100 μM) vs. TEO (300 μL/L): ### p < 0.0001; (C) Control vs. Sco (100 μM): # p < 0.01, Sco (100 μM) vs. TEO (25 μL/L): ## p < 0.001, Sco (100 μM) vs. TEO (150 μL/L): ## p < 0.001, and Sco (100 μM) vs. TEO (300 μL/L): ## p < 0.001; (D) Control vs. Sco (100 μM): # p < 0.01, Sco (100 μM) vs. TEO (25 μL/L): # p < 0.01, Sco (100 μM) vs. TEO (150 μL/L): ### p < 0.0001, and Sco (100 μM) vs. TEO (300 μL/L): ### p < 0.0001.