Thymus vulgaris essential oil (TEO: 25, 150, and 300 μL/L) improved locomotion pattern and memory in the Y-maze test. (A) Representative locomotion tracking pattern of the control, scopolamine (Sco: 100 μM), and TEO (25, 150, and 300 μL/L) treated groups. (B) Represent time spent in each arm (start, novel and novel arm) in different groups. (C) Represent the total distance traveled by zebrafish in the tank in different groups. (D) Represent the turn angle of zebrafish in the tank in different groups. Values are means ± S.E.M. (n = 10). For Tukey’s post hoc analyses: (B) Control vs. Sco (100 µM): ### p < 0.0001, Sco vs. TEO (25 µL/L): ## p < 0.001, Sco vs. TEO (150 µL/L): ### p < 0.0001 and Sco vs. TEO (300 µL/L): ### p < 0.0001; (C) Control vs. Sco (100 µM): ### p < 0.0001, Sco vs. TEO (25 µL/L): ## p < 0.001, Sco vs. TEO (150 µL/L): ## p < 0.001 and Sco vs. TEO (300 µL/L): ## p < 0.001; (D) Control vs. Sco (100 µM): ## p < 0.001; (E) Control vs. Sco (100 µM): ### p < 0.0001, Sco vs. TEO (25 µL/L): ### p < 0.0001, Sco vs. TEO (150 µL/L): ### p < 0.0001 and Sco vs. TEO (300 µL/L): ### p < 0.0001.