Figure 5.
Transcriptome profiles of host immune-response genes. (A) Volcano plot shows the fold change (log2 transformed) and variance for human gene transcripts in AcHERVenv-CMV-GFP-infected 293TT cells relative to uninfected controls. Differentially expressed transcripts are highlighted in orange. (B) Heatmap depicting relative change in the expression of host immune-response genes. (C) qRT-PCR analysis of the indicated genes in AcHERVenv-CMV-GFP-infected 293TT cells and uninfected controls verifies the RNA-seq data. mRNA levels of indicated genes in 293TT and 293TT-N were normalized to GAPDH levels. Gene expression levels of 293TT relative to 293TT-N are shown as the means ± s.e.m. of six independent replicates.