Skip to main content
. 2020 Nov 4;9(11):775. doi: 10.3390/antibiotics9110775

Figure 2.

Figure 2

Figure 2

Visual inspection of 72 h M12 GAS biofilms captured via SEM revealed substantial extracellular polymeric substances (EPS) present in biofilms formed on PNGase F pre-treated pharyngeal cell monolayers. Images are representative of biofilms formed on (A) untreated and (C) PNGase F pre-treated pharyngeal monolayers. GAS biofilms show chained cocci (white arrows) arranged into three dimensional aggregated structures with EPS matrix material present (big and small black arrows). SEM images of (B) untreated and (D) PNGase F pre-treated Detroit 562 pharyngeal cell monolayers (without biofilm) are also included. Biofilms and Detroit 562 pharyngeal cell monolayers (without biofilm) were imaged using the JEOL JSM-7500 microscope at 15,000× and 500× magnification, respectively. SEM images were randomly selected and represent two biological replicates with two technical replicates each.