Table 1.

M12 GAS biofilms exhibit enhanced penicillin tolerance when formed upon exoglycosidase-treated pharyngeal cell monolayers. MBECs (μg/mL) determined for M12 GAS biofilms were compared to the planktonic M12 GAS minimum inhibitory concentration (MIC) (μg/mL). Data represent n = 3 biological replicates, with 3 technical replicates each.

Biofilm Monolayer	M12 MBEC (μg/mL)	Fold Greater Tolerance Compared to MIC
Untreated	62.5	2500
α1-6 mannosidase	125	5000
α1-2,3 mannosidase	125	5000
Sialidase A	125	5000