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. 2020 Nov 4;12(11):3391. doi: 10.3390/nu12113391

Figure 4.

Figure 4

GI transit and mucosal bacterial adherence. (a–d) Weight of small intestine and colon content was determined at day 5 (g/kg bodyweight), 1 h after the last bolus of EN, in separated regions (a) proximal small intestine, (b) mid small intestine, (c) distal small intestine and (d) colon. (e,f) Fraction of piglets that not cleared the contrast fluid from the small intestine over time (h), determined by x-ray analysis, for (e) preterm and (f) near-term piglets. (g) Fluorescent in-situ hybridization (FISH) detection of eukaryotic bacteria in proximal and distal small intestinal tissue slides, with quantification of staining intensity in (h) proximal small intestine and (i) distal small intestine. n = 14 for preterm piglets, n = 8–9 for near-term piglets. white scale bar equals 50 µm. * p < 0.05, ** p < 0.01, ns = non-significant based on Mann–Whitney test between EH-WPC and MP-WPC. SI = small intestine, EUBAC = eubacteria.