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. 2020 Nov 4;8(11):1730. doi: 10.3390/microorganisms8111730

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The olp1Δ mutants are sensitive to 5-fluorocytosine (5-FC) and lithium chloride (LiCl). (A) Capsule formation (top) and melanin production (bottom) of each cryptococcal strain. (B) Statistical analysis of the capsule formation in each Cryptococcus strain. (C) The overexpression of the OLP1 gene was measured by relative qRT-PCR analysis. (D) Growth of each cryptococcal strains on yeast extract peptone dextrose (YPD) or YPD supplemented with various stress reagents. The plates were grown for two days at 30 °C. The name of the strains is indicated on the left and the conditions are indicated at the top.