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. 2020 Nov 4;8(11):1730. doi: 10.3390/microorganisms8111730

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Mating hyphae production and sporulation on the wild-type and olp1Δ mutants. (A) Induction of the mating of wild-type (H99 × KN99a), olp1Δ mutants (TBL337 × TBL348), olp1Δ::OLP1 complementation strains (TBL349 × TBL352), and OLP1^OE overexpression strains (TBL353 × TBL354) on MS media. Mating structures at 40× magnification (top) and 400× magnification (bottom) were incubated at 25 °C in the dark before being photographed. Bar = 10 μm. (B) The development of fungal nuclei in yeast cells, mating hyphae, and basidia of the wild-type (TBL101 × TBL102, top) and olp1Δ mutants (TBL371 × TBL372, bottom). After incubating on MS medium in the dark for 7 or 14 days, the mating cultures were isolated and visualized by confocal microscopy (Olympus, FV1200). DIC: differential interference contrast; Bars, 5 µm.