Table 2.
Primers used in this study.
| Primers | Targeted Genes | Sequence (5′-3′) |
|---|---|---|
| TL17 | M13F | GTAAAACGACGGCCAG |
| TL18 | M13R | CAGGAAACAGCTATGAC |
| TL19 | NEO split F | GGGCGCCCGGTTCTTTTTGTCA |
| TL20 | NEO split R | TTGGTGGTCGAATGGGCAGGTAGC |
| TL59 | NEO R4 | TGTGGATGCTGGCGGAGGATA |
| TL67 | STE20A α F | CCAAAAGCTGATGCTGTGGA |
| TL68 | STE20A α R | AGGACATCTATAGCAGAT |
| TL69 | STE20Aa F | TCCACTGGCAACCCTGCGAG |
| TL70 | STE20Aa R | ATCAGAGACAGAGGAGCAAGAC |
| TL217 | GAPDH qRT-PCR F | TGAGAAGGACCCTGCCAACA |
| TL218 | GAPDH qRT-PCR R | ACTCCGGCTTGTAGGCATCAA |
| TL404 | OLP1 KO F1 | CTCCCCAGACAAGCACATTCC |
| TL405 | OLP1 KO R1 | CTGGCCGTCGTTTTACGACGCGTCTACACCACTCAGCAA |
| TL1253 | OLP1 KO F2 | GTCATAGCTGTTTCCTGCAAGATTCTGTGCGTATGGTGTGC |
| TL1254 | OLP1 KO R2 | GTTTGTTCTTTTGGCGGGTTTGAG |
| TL1255 | OLP1 KO F3 | ATATGAATTGCTGCGTGTGACC |
| TL1256 | OLP1 KO R3 | GCTTATGCTCCTTCTTCCAGTATT |
| TL410 | OLP1 KO F4 | TCCAAAGAAGAAGACAGCAACC |
| TL515 | OLP1 mCherry F | TTAGTAAACTCGCCCAACATGTCTGGATCCATGCCTATTCACACTCTTGCTTC |
| TL516 | OLP1 mCherry R | CTTGCTCACCATTCTAGAACTAGTGGATCCTTTGCCCTCTGGCTTGGTTCTG |
| TL539 | OLP1 QPCR F1 | TACGAGCCTCTCGACGATAC |
| TL540 | OLP1 QPCR R1 | TCTGGCTTGGTTCTGTCTTTAC |
| TL556 | OLP1 Comp F | GATATCGAATTCCTGCAGCCCGGGGGATCCTCCAAAGAAGAAGACAGCAACCTA |
| TL1257 | OLP1 Comp R | CGGTGGCGGCCGCTCTAGAACTAGTGGATCTTTGCCTACAGGATTTTGGTCACT |
| TL727 | OLP1 Pro-OLP1 F1 | TCGACGGTATCGATAAGCTTCGCCGACGACCAAGATACAG |
| TL729 | OLP1 Pro-OLP1 R1 | ATTCTAGAACTAGTGGATCCTTTGCCCTCTGGCTTGGTTCTGTC |
Note: a, a mating type.