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. 2020 Nov 4;11(11):1309. doi: 10.3390/genes11111309

Figure 2.

Figure 2

TNF-α activated the NF-κB signaling pathway in HLEC. (A,B) Changes in the expression of IKKα, p-IKKα, IκBα, and p-IκBα proteins following treatment with TNF-α (10, 20, and 40 ng/mL) for the 48-h time point in HLEC. Western blotting was performed to detect expression changes of the listed proteins, using β-actin as loading controls. Data represent the mean ± S.E.M. from three independent experiments. (C,D) After HLEC was treated with TNF-α (10, 20, and 40 ng/mL) for the 48-h time point, the cytoplasmic/membrane and nuclear fractions of the HLEC were analyzed by Western blotting for the NF-κB nuclear translocation, with β-actin and Lamin A as cytoplasmic and nuclear loading controls, respectively. Data represent the mean ± S.E.M. from three independent experiments. Asterisks denote statistically significant (* p < 0.05 and ** p < 0.01) differences compared with controls by one-way ANOVA.