Figure 4.

Optimization plots of factors vs. responses, where each column describes the effect of individual factors of the regression Equations (1)–(4) on the measured responses α and tR_Last. Optimum conditions that simultaneously achieve maximum α and minimum tR_Last are 5.4511 mM HP-β-CD and 52.250 mM buffer in a global desirability (D) of 96.90%. Lines and values in red show the factor points included in the optimized response conditions, Horizontal blue lines shows the desirability function values achieving maximum α and minimum tR_Last.