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. 2020 Nov 27;20:572. doi: 10.1186/s12935-020-01666-1

Fig. 5.

Fig. 5

CUL4B is a target of miR-942-5p. a The predicted binding site between miR-942-5p and CUL4B 3′UTR was shown. The binding relationship between miR-942-5p and CUL4B 3′UTR was confirmed by dual-luciferase reporter assay (b, c) and RIP assay (d, e). f, g The mRNA and protein levels of CUL4B were measured in OV90 and SKOV3 cells transfected with miR-NC, miR-942-5p, anti-miR-NC or anti-miR-942-5p. h, i The mRNA and protein levels of CUL4B were examined in OC tissues and normal tissues. j, k The expression of CUL4B in IOSE80, OV90 and SKOV3 cells was determined by qRT-PCR and Western blot. *P < 0.05