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. 2020 Nov 5;12(11):3269. doi: 10.3390/cancers12113269

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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TSPAN1 inhibition induces apoptosis in HNSCC cells. (A) Western blot analysis of TSPAN1 and PARP1 cleavage in parental and CDDP-resistant HNSCC cells upon TSPAN1 depletion. In the CCL-138-R cell line, despite not observing TSPAN1 inhibition at protein level (but it was inhibited at the mRNA level), an induction of PARP1 was observed. Note: the lower band corresponds to cleaved PARP1 (arrow). Vinculin was used for protein loading normalization. (B) Apoptosis analysis by FACS using Annexin V expression kit. Representative scatter plots of propidium iodide (y-axis) vs. Annexin V-APC (x-axis) to detect early (right bottom quadrant) and late (right upper quadrant) apoptosis in parental and their respective CDDP-resistant cells (-R) JHU029, HTB-43 and CCL-138, comparing NC vs. TSPAN1depleted cells.