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. 2020 Nov 5;12(11):3270. doi: 10.3390/cancers12113270

Figure 4.

Figure 4

Validation of concordantly and discordantly expressed differentially expressed proteins and transcripts in grade III vs. grade I meningiomas. (a) Western blot (WB) analysis of the proteins MAOB, FABP7 and RBP1 that demonstrated a concordant increase in expression following transcriptome–proteome integration in meningioma tissue lysates. WB quantification of average densities of MAOB, FABP7 and RBP1 in grade III meningioma compared to average densities in grade I is shown. GAPDH was used as loading control. Grade I n = 5, grade III n = 5. (b) Immunohistochemistry validation of MAOB, FABP7 and RBP1. Representative images show immunohistochemical staining in grade I and grade III meningioma. Table S5 details the full list of specimens and corresponding semi-quantitative assessment. Grade I n = 10, grade III n = 10. RT-qPCR validation of discordantly expressed transcripts (c) CST3 (d) LAMP2 (e) PACS1 and (f) HTRA1 that exhibited negative transcript but positive protein LFC in grade III meningioma compared to grade I following transcriptome–proteome integration. Grade I n = 7, grade III n = 7. Statistical significance was determined by Mann–Whitney test * p ≤ 0.05; *** p ≤ 0.001.