Table 1.
Identification of protein bands excised from the sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of oleosome proteins extracted with 2% SDS buffer (see Figure 2) and analyzed by Liquid Chromatography High-Resolution Tandem Mass Spectrometry (LC-HR-MS/MS) through detection of the prototypic peptides. All relevant parameters retrieved by software analysis are reported.
| Band | Accession | Description (OS Prunus dulcis) |
Coverage (%) | Peptides (Unique) | PSMs | MW (kDa) | Score |
|---|---|---|---|---|---|---|---|
| 1 | A0A5E4EAH6 | PREDICTED: basic 7S globulin | 58.7 | 21 (21) | 107 | 46.9 | 70.2 |
| 2 | A0A5E4EKE0 | PREDICTED: peroxygenase | 15.9 | 3 (2) | 13 | 26.5 | 5.9 |
| A0A4Y1QWY8 | Beta-tonoplast intrinsic protein | 8.9 | 2 (2) | 8 | 27.2 | 2.7 | |
| 3 | A0A5E4FFS0 | PREDICTED: legumin | 8.5 | 4 (4) | 14 | 63 | 5.6 |
| A0A4Y1QWY8 | Beta-tonoplast intrinsic protein | 23.4 | 4 (4) | 15 | 27.2 | 4.4 | |
| 4 | A0A5E4ET55 | Oleosin | 50.6 | 5 (4) | 27 | 16.6 | 9.5 |
| A0A5E4FFS0 | PREDICTED: legumin | 5.6 | 2 (2) | 4 | 63 | 1.3 | |
| A0A5E4EAT1 | Oleosin | 52.0 | 4 (4) | 16 | 15.6 | 4.1 |