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. 2020 Nov 5;12(11):3267. doi: 10.3390/cancers12113267

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Transcriptional profiles of differentiating hTERT-MSCs: effects of Gln and Asn. hTERT-MSCs were differentiated in osteogenic media (ASC/DEX +) for 14 days with 2 mM Gln (+ Gln), without Gln (−Gln) or without Gln in the presence of 380 µM Asn (−Gln + Asn). (A–I) Bar graphs represent the median gene expression levels of selected genes significantly modulated by both Gln and Asn (A–D), exclusively by Gln (E–G) and Asn (H,I). (J) Heatmap of selected genes differently expressed in hTERT-MSCs differentiated with 2 mM Gln (+ Gln), without Gln (−Gln), and without Gln in the presence of 380 µM Asn (−Gln + Asn).