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. 2020 Nov 27;11:512. doi: 10.1186/s13287-020-02029-3

Table 1.

Primer sequences using reverse transcription polymerase chain reaction

Genes Primer sequences Tm
Oct4 Forward 5′-AGTGAGAGGCAACCTGGAGA-3′ 52
Reverse 5′-GTGAAGTGAGGGCTCCCATA-3′
Nanog Forward 5′-TTCTTGACTGGGACCTTGTC-3′ 52
Reverse 5′-GCTTGCCTTGCTTTGAAGCA-3′
Sox2 Forward 5′-GGGCAGCGTGTACTTATCCT-3′ 52
Reverse 5′-AGAACCCCAAGATGCACAAC-3′
HLA-G Forward 5′-GCGGCTACTACAACCAGAGC-3′ 58
Reverse 5′-GCACATGGCACGTGTATCTC-3′
TERT Forward 5′-GAGCTGACGTGGAAGATGAG-3′ 55
Reverse 5′-CTTCAAGTGCTGTCTGATTCCAATG-3′
Albumin Forward 5′-TGAGTTTGCAGAAGTTTCCA-3′ 60
Reverse 5′-CCTTTGCCTCAGCATAGTTT-3′
TAT Forward 5′-AACGATGTGGAGTTCACGG-3′ 55
Reverse 5′-GACACATCCTCAGGAGAATGG-3′
HNF1A Forward 5′-TACACCACTCTGGCAGCCACACT-3′ 60
Reverse 5′-CGGTGGGTACATACCTGACAGAAC-3′
CYP2B6 Forward 5′-CTTGACCTGCTGCTTCTTCC-3′ 55
Reverse 5′-TGCTTCCCGCCTCAGATTTCTC-3′
CYP3A4 Forward 5′-ATCATTGCTGTCTCCAACCTTCAC-3′ 60
Reverse 5′-TGCTTCCCGCCTCAGATTTCTC-3′
β-actin Forward 5′-TCCTTCTGCATCCTGTCAGCA-3′ 58
Reverse 5′-CAGGAGATGGCCACTGCCGCA-3′