Skip to main content
. 2020 Nov 27;11:512. doi: 10.1186/s13287-020-02029-3

Table 3.

Rat primer sequences using quantitative real-time polymerase chain reaction

Genes Primer sequences Tm
PGC-1α Forward 5′-GTGCAGCCAAGACTCTGTATGG-3′ 60
Reverse 5′-GTCCAGGTCATTCACATCAAGTTC-3′
NRF1 Forward 5′-GCTGTCCCACTCGTGTCGTAT-3′ 60
Reverse 5′-GTTTGAGTCTAACCCATCTATCCG-3′
mtTFA Forward 5′-CGCCTAAAGAAGAAAGCACA-3′ 60
Reverse 5′-GCCCAACTTCAGCCATTT-3′
mtD-Loop Forward 5′-GGTTCTTACTTCAGGGCCATCA-3′ 55
Reverse 5′-GATTAGACCCGTTACCATCGAGAT-3′
β-actin Forward 5′-GGGATGTTTGCTCCAACCAA-3′ 55
Reverse 5′-GCGCTTTTGACTCAAGGATTTAA-3′
GAPDH Forward 5′-TCCCTCAAGATTGTCAGCAA-3′ 60
Reverse 5′-AGATCCACAACGGATACATT-3′