Fig. 5.

Prevention of photoreceptor cell death by the IL-1R modulator rytvela. a Spider-graph quantification of ONL thickness on DAPI-stained retinal sections from non-illuminated animals (control) and from blue light–exposed mice treated with vehicle, rytvela, or Kineret. Statistical analysis was performed using the area under the curve values (to assess photoreceptor density). Data are expressed as mean ± SEM and analyzed using one-way ANOVA with Holm-Sidak correction for multiple comparisons; n = 4–5. **p < 0.01, *p < 0.05. ONL: outer nuclear layer; AUC: area under the curve. b Representative images of photoreceptor cone outer and inner segments using fluorescein-PNA (green)-stained retinas. Scale bar 50 μm. The graph illustrates the quantitative analysis of cone segment length. c Representative images of TUNEL (green)-stained retinas in control and blue light-exposed mice administrated with vehicle or rytvela. The graph illustrates the quantitative analysis of TUNEL-positive cells in the ONL. Scale bar 50 μm. Data are expressed as mean ± SEM and analyzed using one-way ANOVA with Holm-Sidak correction for multiple comparisons; n = 6 per group. ****p < 0.0001