Table 5.
Biofilm eradication activity of EOs against MRSA isolates.
Isolates | Control OD570 ± SD | O. majorana OD570 ± SD | Eradication (%) | T. zygis OD570 ± SD | Eradication (%) | R. officinalis OD570 ± SD | Eradication (%) |
---|---|---|---|---|---|---|---|
1 | 0.418 ± 0.024 | 0.417 ± 0.015 | 0 | 0.415 ± 0.019 | 0 | 0.413 ± 0.021 | 0 |
2 | 3.13 ± 0.096 | 1.754 ± 0.055 | 43.96 | 1.626 ± 0.06 | 48.05 | 2.709 ± 0.084 | 13.45 |
3 | 4.362 ± 0.086 | 2.92 ± 0.071 | 33.05 | 3.468 ± 0.091 | 20.49 | 2.15 ± 0.079 | 50.71 |
4 | 1.939 ± 0.078 | 1.941 ± 0.069 | 0 | 1.931 ± 0.074 | 0 | 1.115 ± 0.063 | 42.49 |
5 | 1.41 ± 0.088 | 0.237 ± 0.025 ** | 83.19 | 0.079 ± 0.008 *** | 94.39 | 0.103 ± 0.011 ** | 92.69 |
6 | 0.176 ± 0.016 | 0.078 ± 0.005 * | 55.68 | 0.061 ± 0.004 * | 65.34 | 0.131 ± 0.018 | 25.56 |
7 | 0.189 ± 0.012 | 0.185 ± 0.013 | 0 | 0.191 ± 0.015 | 0 | 0.186 ± 0.015 | 0 |
8 | 2.465 ± 0.083 | 0.37 ± 0.045 ** | 84.98 | 2.153 ± 0.079 | 12.65 | 1.143 ± 0.059 | 53.63 |
9 | 0.304 ± 0.056 | 0.308 ± 0.021 | 0 | 0.309 ± 0.022 | 0 | 0.302 ± 0.024 | 0 |
10 | 1.722 ± 0.066 | 0.386 ± 0.027 ** | 77.58 | 1.074 ± 0.055 | 37.63 | 0.174 ± 0.014 ** | 89.89 |
11 | 0.269 ± 0.013 | 0.262 ± 0.016 | 0 | 0.265 ± 0.025 | 0 | 0.271 ± 0.02 | 0 |
12 | 2.334 ± 0.073 | 0.687 ± 0.032 ** | 70.56 | 1.356 ± 0.058 | 41.90 | 2.328 ± 0.026 | 0 |
13 | 0.433 ± 0.026 | 0.431 ± 0.026 | 0 | 0.436 ± 0.031 | 0 | 0.43 ± 0.032 | 0 |
14 | 2.617 ± 0.078 | 0.052 ± 0.003 *** | 98.01 | 2.614 ± 0.082 | 0 | 2.609 ± 0.079 | 0 |
16 | 0.286 ± 0.034 | 0.287 ± 0.02 | 0 | 0.281 ± 0.015 | 0 | 0.288 ± 0.019 | 0 |
17 | 1.249 ± 0.028 | 1.247 ± 0.063 | 0 | 1.241 ± 0.068 | 0 | 1.244 ± 0.064 | 0 |
18 | 0.298 ± 0.011 | 0.292 ± 0.018 | 0 | 0.294 ± 0.016 | 0 | 0.296 ± 0.023 | 0 |
19 | 0.676 ± 0.026 | 0.679 ± 0.039 | 0 | 0.671 ± 0.029 | 0 | 0.674 ± 0.038 | 0 |
20 | 0.894 ± 0.045 | 0.448 ± 0.028 | 49.88 | 0.613 ± 0.036 | 31.43 | 0.584 ± 0.034 | 34.67 |
21 | 0.516 ± 0.068 | 0.514 ± 0.04 | 0 | 0.513 ± 0.031 | 0 | 0.517 ± 0.041 | 0 |
22 | 0.319 ± 0.022 | 0.313 ± 0.024 | 0 | 0.317 ± 0.024 | 0 | 0.314 ± 0.021 | 0 |
23 | 1.194 ± 0.039 | 1.19 ± 0.052 | 0 | 1.195 ± 0.057 | 0 | 1.192 ± 0.051 | 0 |
24 | 0.808 ± 0.027 | 0.801 ± 0.048 | 0 | 0.805 ± 0.044 | 0 | 0.81 ± 0.046 | 0 |
25 | 2.249 ± 0.069 | 2.245 ± 0.069 | 0 | 1.824 ± 0.072 | 18.89 | 1.806 ± 0.058 | 19.69 |
26 | 3.396 ± 0.094 | 1.674 ± 0.049 | 50.70 | 1.785 ± 0.058 | 47.43 | 2.025 ± 0.06 | 40.37 |
27 | 0.568 ± 0.021 | 0.561 ± 0.034 | 0 | 0.567 ± 0.039 | 0 | 0.564 ± 0.039 | 0 |
28 | 0.535 ± 0.019 | 0.437 ± 0.025 | 18.31 | 0.337 ± 0.03 | 37 | 0.081 ± 0.006 * | 84.85 |
29 | 0.133 ± 0.011 | 0.134 ± 0.019 | 0 | 0.136 ± 0.026 | 0 | 0.132 ± 0.011 | 0 |
30 | 2.989 ± 0.083 | 0.348 ± 0.023 ** | 88.35 | 1.342 ± 0.056 | 55.10 | 1.756 ± 0.053 | 41.25 |
ATCC 25922 | 4.32 ± 0.098 | 2.796 ± 0.085 | 35.27 | 1.972 ± 0.068 | 54.35 | 2.577 ± 0.087 | 40.34 |
* Isolates changed from low-grade positive to biofilm negative after treatment with EOs. ** Isolates changed from highly positive to low-grade positive after treatment with EOs. *** Isolates changed from highly positive to biofilm negative after treatment with EOs.