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. 2020 Nov 5;12(11):1258. doi: 10.3390/v12111258

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunoreactivity of peptGL^ZIKALIVax mutants with anti-ZIKALIVax immune serum. The O.D₄₅₀ or signal intensity of individual anti-ZIKALIVax immune sera in relation to peptGL^ZIKALIVax and its mutants at positions 8 and 12 (A) or 14 (B) measured through peptide-based ELISA. Peptides at concentration of 3 µg mL⁻¹ were incubated with individual anti-ZIKALIVax immune sera (n = 7) (ZIKALIVax serum) showing the higher reactivity with peptGL^ZIKALIVax at dilution 1:200 (Figure 2B). Serum of mice (n = 7) that received heat-inactivated ZIKALIVax at dilution 1:200 served as a control serum. Each closed circle represents an individual mouse.