Figure 9.

Recognition of rEDI^ZIKALIVax in relation with peptGL^ZIKALIVax-reactive antibody. HEK-293T cells were transfected 24 h with plasmids expressing rEDI^ZIKALIVax or its mutants or mock-transfected (mock). FACS analysis was performed to detect expression of rEDI in transfected cells. For FACS analysis, cells were incubated with anti-FLAG antibody (A) or KLH-peptGL^MR766 immune serum S1-S1-RE (KLH-peptGL^MR766 serum) (B) as primary antibody and then Alexa 488-conjugated anti-mouse IgG antibody as secondary antibody. The MFI values of FITC were determined. The error bars represent standard errors of two independent experiments. In (A), MFI values generated by the three rEDI^ZIKALIVax mutants in comparison with rEDI^ZIKALIVax. In (B), MFI values generated by rEDI^ZIKALIVax and its mutants in comparison with mock. Pairwise comparisons were performed and statistically significant comparisons are shown as *** p < 10⁻², ** p< 10⁻¹, * p < 0.05. The nonstatistically significant comparisons are not indicated.