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. 2020 Nov 5;9(11):2422. doi: 10.3390/cells9112422

Figure 4.

Figure 4

In vitro and in vivo differentiation of marmoset iPSCs. (A,B) Neurons positive for β-III-Tubulin and NESTIN. (C) Endodermal differentiation with cobblestone-like morphology. (D) Primitive endoderm stained with anti-alpha Fetoprotein (AFP). (E–H) Cardiomyocytes stained for: (E) cTnT, (F) MLC2a, (G) α-actinin and CX43 (arrowheads), and (H) Titin. (I–Q) Teratoma assay: (I) Gut-like endoderm. (J) Gland-like endoderm. (K) Bone. (L) Bone tissue stained for SOX17 (which is also involved in osteogenesis). (M) Gut-like tissues stained with anti-SOX9. (N) Smooth muscle stained for smooth muscle actin (SMA). (O) Neuronal-like cells stained for β-III-Tubulin. (P,Q) Successive sections stained for presence of NESTIN and PAX6. (Scale bars: (A,B,D–H) = 50 um; (C) = 200 μm; (I–Q) = 100 μm).