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. 2020 Nov 5;9(11):2422. doi: 10.3390/cells9112422

Figure 6.

Figure 6

Role of small molecule inhibitors in maintaining pluripotency gene expression of marmoset iPSCs in long-term culture. (A) Marmoset iPSCs cultured either with full culture medium, with omission of individual factors (-IWR1, -CHIR99021, -CGP77675, -hrLIF, -Forskolin), or without any inhibitors and hrLIF. (All scale bars = 50 μm). (B) Pluripotency gene expression of marmoset iPSCs cultured without different inhibitors or hrLIF for 5–6 passages determined by relative quantitation qPCR. One of the lines cultured without any inhibitors and hrLIF was used as a reference. (Data are presented as mean + SEM. Statistically significant differences between experimental groups are indicated with asterisks as follows: * p < 0.05, ** p < 0.01).