Skip to main content
. 2020 Nov 24;8:e10374. doi: 10.7717/peerj.10374

Figure 1. Wnt16 expression and function in osteogneisis of periosteum derived cells (PDCs).

Figure 1

Wnt16 expression and function in osteogneisis of periosteum derived cells (PDCs). (A) Flow cytometry showed that approximately 89.1% in the third passage of the isolated PDCs were CD90+/CD105-, and approximately 66.6% of them were Sca-1+/CD45-. Within CD90+/Sca-1+, 87.2% cells wereCD90-/CD45-. (B) Change of Wnt16 mRNA expression in PDCs under osteogenesis induction medium for 14 days (n = 3). (C) mRNA of Wnt16, Runx2, OCN and collagen types I (Col-1) after Wnt16 knocking down (kd) by using wnt16 shRNA in PDCs after 7 days of osteogenic indction (n = 3). (D) Whole plate mount of alkaline phosphataseactivity in PDCs with or without Wnt16 knocking down after 7 days of osteogenic indction. (E) Whole plate mount of Alizarin Red staining image in PDCs after 21 days of induction. mRNA of Wnt16 (F), Runx2 and OCN (H) upon Wnt16 overexpression (OE) by using lentivirus vector after 2 days of induction. (n = 3) (G) Runx2 and Col-1 protein upon Wnt16 overexpression. * p < 0.05, ** p < 0.001, *** p < 0.0001.