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. 2020 Nov 24;8:e10374. doi: 10.7717/peerj.10374

Figure 4. Wnt16 activate canonical wnt signaling pathway.

Figure 4

(A–F) immunofluorescence staining of β-catenin in Wnt16 oeverxpressed (OE) PDCs or in control groups. Overexpression of Wnt16 in PDCs promote β-catenin translocating into nucles. (G) TCF/LEF promoter relative activity was quantified by transfecting the TCF/LEF reporter and using a luciferase assay in Wnt16 OE cells added with PBS, Wnt3a or DKK-1 (n = 3). (H) NFAT-dependent transcriptional activity was quantified by transfecting the NFAT reporter and using a luciferase assay in Wnt16 OE cells added with PBS, Wnt5a or INCA-6 (n = 3). Scale bar: 100 µm, * p < 0.05.