Figure S3.

SFR deficiency alters the GC reaction and interferes with bone marrow chimeras. Related to Fig. 7. (A–C) Same as Fig. 7, E–G, except that mean fluorescence intensity (MFI) of CD95 on total B cells was measured on day 7 (A); numbers of CD4⁺, Tfh cells, and GC Tfh cells were determined on day 7 (B); and MFIs of PD-1 on CD4⁺ cells plus numbers of PD-1⁺CD4⁺ cells were analyzed (C; WT, n = 4; SFR KO, n = 5). Data are from one experiment. (D–F) Bone marrow chimeras were created using bone marrow cells from WT (CD48⁺) and SFR KO (CD48⁻) mice. The experiment was performed as detailed in D. After reconstitution and immunization, the ratios of SFR KO to WT cell numbers in CD19⁺ and GC B cells (E) or CD4⁺, Tfh, and GC Tfh cells (F) were determined. Data are pooled from two experiments. Chimeric mice, n = 10. Each symbol represents one mouse. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 (two-tailed Student’s t test). Data are shown as mean ± SEM. BMC, bone marrow cell; D, day; ns, not significant.