Figure 3.

Geographical and tissue distribution of AealbAV and phylogenetic relationship of AealbAV strains. (a) AealbAV is present worldwide in Ae. albopictus laboratory colonies and wild-caught mosquitoes. Locations of mosquito collection from RNA-Seq and miRNA-Seq libraries that harbor AealbAV are shown. Red up-pointing triangles refer to the location of the laboratory colonies; Red down-pointing triangles refer to the collection sites of the samples used to establish the laboratory colonies (if this information was available). An identical symbol nearby the triangles specifies the link between collection and colony sites (also see Table 1); Black points refer to the collection sites of wild-caught mosquitoes. (b) Phylogeny of representatives AealbAV genomes from different geographical locations. AealbAV genomes cluster by the geographical origin of their mosquito hosts. AealbAV strain from Thailand is the most differentiated. The unrooted Maximum likelihood phylogeny was constructed from alignments of nucleotide sequences of AealbAV strains using a GTR + F + I model with 1000 bootstraps. Node values correspond to bootstrap support. Branch lengths represent expected numbers of substitutions per nucleotide site. (c) AealbAV sequences were found in Ae. albopictus libraries derived from various life stages including embryos/eggs, and tissues, including oocytes and ovaries.