Table 1.
Details of the Ae. albopictus RNA-Seq and miRNA-Seq libraries in which AealbAV was identified.
| SRA Study | Number of Libraries | Origin | Lab Colony Location | Sampling Year for Colony Establishment | Collection Site | Sampling Time/(Lab Colony Generation) | Library Type | Library Layout | Evidence | Max Depth of Coverage (% Breadth of Coverage) | Tissue | Sex |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SRP012105 | 12 | Lab colony | Georgetown University (USA) | 2008 | Manassas (USA) | -/(F10) | RNA-Seq | Paired (Illumina) | Assembled genomes | 361.5 (100) | Embryos | Mixed |
| SRP018112 | 17 | Lab colony | Georgetown University (USA) | 2008 | Manassas (USA) | -/(F13) | RNA-Seq | Paired (Illumina) | Assembled genomes | 57.4 (100) | 1st instar pharate larvae (whole eggs) | Mixed |
| SRP050258 | 16 | Lab colony | Georgetown University (USA) | 2013 | Manassas (USA) | 2013/(F3) | RNA-Seq | Paired (Illumina) | Assembled genomes | 27.7 (100) | Adult whole body | Female |
| SRP096579 | 8 | Lab colony | Georgetown University (USA) | 2010 | Manassas (USA) | -/(F12) | miRNA-Seq | Single (Illumina) | Mapped reads | 2.6 (73) | Whole Eggs (Pharate Larvae) | Mixed |
| SRP007714 | 2 | Lab colony | Georgetown University (USA) | 2008 | Manassas (USA) | 2010/(F5) | RNA-Seq | Single (454) | Mapped reads | 4.0 (64) | Oocytes | Female |
| SRP228299 * | 2 | Lab colony | Institut Pasteur (FRA) | 2008 | Kawasaki (JPN) | 2015/- | miRNA-Seq | Single (Illumina) | Mapped reads | 49.8 (99) | Whole body, ovaries | Female |
| SRP071220 | 8 | Lab colony | Sapienza University (ITA) | 2012 | Rome (ITA) | 2013/- | RNA-Seq | Paired (Illumina) | Assembled genomes | 169.3 (100) | Whole body, heads, antennae, maxillary palps | Male/Female |
| SRP056407 | 20 | Lab colony (MRA-804) | The Ohio State University (USA) | na | Florida (USA) | 2012/- | RNA-Seq | Paired (Illumina) | Assembled genomes | 33.6 (100) | Malpighian tubules | Female |
| SRP034701 | 18 | Lab colony (MRA-804) | The Ohio State University (USA) | na | Florida (USA) | 2012/- | RNA-Seq | Single (Illumina) | Mapped reads | 3.7 (85) | Malpighian tubules | Female |
| SRP008316 ** | 5 | Lab colony | Fralin Life Science Institute (USA) | na | na | 2012/- | miRNA-Seq | Single (Illumina) | Assembled genomes | 1228.7 (100) | Whole body, head and thorax | Female |
| SRP188743 | 9 | Wild-caught | - | - | Guangzhou (CHN) | 2017 | miRNA-Seq | Single (Illumina) | Mapped reads | 4.8 (77) | Adult Whole body, larvae | Mixed |
| PRJNA602498 | 1 | Wild-caught | - | - | Arco (ITA) | 2011 | RNA-Seq | Paired (Illumina) | Assembled genomes | 129.4261 (100) | Antennae | Mixed |
| PRJNA602498 | 1 | Wild-caught | - | - | Trento (ITA) | 2011 | RNA-Seq | Paired (Illumina) | Assembled genomes | 512.9825 (100) | Antennae | Mixed |
| PRJNA602498 | 1 | Wild-caught | - | - | Ban Rai (THA) | 2012 | RNA-Seq | Paired (Illumina) | Assembled genomes | 27.8252 (100) | Antennae | Mixed |
| SRP266553 | 14 | Wild-caught | - | - | Ticino (CHE) | 2019 | DNA-Seq | Single (Illumina) | Assembled genomes | 21.5 (100) | Adult whole body | Mixed |
AealbAV was identified in several samples from laboratory colonies and wild-caught mosquitoes worldwide. “Number of libraries” corresponds to the number of libraries harboring AealbAV in the corresponding SRA study (See Supplementary Table S1 for details of each library); “Origin” indicates the provenance of the mosquito samples, either a laboratory colony or wild-caught. “Lab colony location” corresponds to the location of the mosquito colony (only for laboratory colonies); “Sampling year for colony establishment” indicates the year in which the source material for establishing the colony was collected, if this information was available (only for laboratory colonies); “Collection Site” corresponds to the sampling site for wild-caught samples. For laboratory colonies, it corresponds to the collection site of the wild source samples used for establishing the colony. “Sampling time/(lab colony generation)” refers to the sampling time for generating the sequencing libraries. The number of generations of the laboratory colonies is reported in brackets if known. “Library type” and “Library layout” indicate the sequencing library type and layout. “Evidence” indicates whether AealbAV reads from AealbAV positive libraries were de novo assembled into contigs or only mapped to the AealbAV representative genome. “Max depth of coverage (% breadth of coverage)” indicates the max depth of coverage found among the individual libraries and the corresponding breadth of coverage, which is reported in brackets; “Tissue” and “Sex” indicate the body tissues/life stage and the sex of the mosquito samples. Multiple tissues are listed if distinct libraries within the same study derived from different tissues (i.e., they are not pools of different tissues). “na” indicates that the information was unavailable. * samples experimentally infected with 3 insect-specific viruses. ** samples experimentally infected with chikungunya virus (CHIKV).