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. 2020 Nov 6;8(11):480. doi: 10.3390/biomedicines8110480

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Identification of 16:1-derived branched fatty acid esters of hydroxy fatty acids (FAHFAs). The cells were stimulated with mannan plus laminarin (0.5 mg/mL each) for the indicated times. Then, 16:1-containing FAHFAs were determined by LC/MS. Three species were detected: hydroxyhexadecenoic acid (16:1/OH-16:1) (cyan), hydroxypalmitic acid (16:1/OH-16:0) (blue) and hydroxystearic acid (16:1/OH-18:0) (dark blue). A representative experiment is shown, and the data are expressed as mean values ± of three individual replicates. * p < 0.05, significantly different from unstimulated cells.