FIGURE 4.

Reactive oxygen species (ROS) and callose accumulation in wild‐type (WT) and pme17 mutants in response to Botrytis cinerea. (a) Photomicrographs indicating callose deposition at the level of the lesion area produced on pme17 mutants and WT plants at 48 hr postinoculation (hpi) with B. cinerea. (b) Callose deposits were quantified per microscopic field using ImageJ. Values are means ± SD (n = 3). (c) ROS accumulation in B. cinerea and mock‐treated leaves at 48 hpi. Leaves were stained with 3,3′‐diaminobenzidine (DAB). Similar results were obtained in three independent experiments. (d) Quantification of DAB intensity using ImageJ. Values are means ± SD (n = 3). Different letters indicate datasets significantly different according to analysis of variance followed by Tukey's test (p < .05)