Figure 2.

Schematic of laminar distribution at lumbar segment 5 (L5). (A) Intensity of WFA staining in the spinal cord grey matter. (B) A significant difference was observed between 67dpi groups and intact, 7d, and 21dNT groups. (C) Number of PNNs in the intact and 67dpi groups. Laminae I, II, III, and X demonstrated the lowest number of PNNs. However, there were no significant differences between groups for any lamina. Γ-MNs do not express PNNs: (D–G) motoneurons in the ventral grey matter labeled with ChAT (green); only α-MNs co-labeled with NeuN (red), whereas γ-MNs (arrow) are NeuN-. (E,F) α-MNs are clearly enveloped by a perineuronal net (WFA), but γ-MNs do not express any organized perineuronal net (F, image shown in black and white for clear visualization). * = p < 0.05. TX Non TR = Transected, non-trained; TX TR = Transected trained.