Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 27;10:139. doi: 10.1186/s13578-020-00499-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 4 — KLHL18 interacts with and promotes the ubiquitination and degradation of PI3Kp85α. a Co-IP of KLHL18 with PI3KP85α in NCI-A549 and NCI-H1299 cells. b Immunofluorescence assay; KLHL18 co-localized with PI3KP85α in NCI-A549 and NCI-H1299 cells. c KLHL18 protein co-immunoprecipitated with CUL3 protein in HEK-293 T cells. d, e KLHL18 inhibited the expression of PI3Kp85α via the Rbx1–CUL3–KLHL18 complex in d NCI-A549 and (E) NCI-H1299 cell lines. The lower graph corresponds to the gray value statistical graph, *P < 0.05, **P < 0.01. f Silencing KLHL18 protein inhibited the ubiquitination of PI3Kp85α protein. g An increase in KLHL18 protein expression levels promoted the ubiquitination-mediated degradation of PI3Kp85α protein