Fig. 7.

The BTB and non-BTB domains of KLHL18 are critical for PI3Kp85α ubiquitination and PD-L1 expression. a The effect of KLHL18 and its splice variants on the ubiquitination level of PI3Kp85α protein in NCI-A549 cells. b The effect of the KLHL18 splice variants on the ubiquitination level of PI3Kp85α protein in NCI-H1299 cells. c, d In NCI-A549 and NCI-H1299 cell lines, decreased PD-L1 protein levels were reversed with the deletion of the BTB domain or amino acids 140–571 near the C-terminus. The lower graph corresponds to the gray value statistical graph, *P < 0.05, **P < 0.01