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. 2020 Nov 7;12(11):1272. doi: 10.3390/v12111272

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Multi-step growth curves of aquatic bird bornavirus 1 (ABBV-1) in primary embryonic fibroblasts from chicken (CEF), duck (DEF), and goose (GEF). Cells were infected at a multiplicity of infection (MOI) of 0.01, and at 3, 5, 9, 11, 13, 15, 17, 19, and 21 days post infection (dpi) a portion of infected cells was fixed and virus was detected using immunofluorescence. Cells were counterstained with DAPI to determine percentage of infected cells over the total. Each data point represents mean +/− standard deviation (n = 3).