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. 2020 Nov 7;12(11):3297. doi: 10.3390/cancers12113297

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Isolation of PpIX-positive EVs from patient plasma. (A) Centrifugation protocol for the isolation of platelet-free plasma from whole blood. (B) hFC analysis of platelet-free plasma. (C) hFC analysis of platelet-free plasma spiked with 2 × 10⁷ U87 derived PpIX-positive EVs. (D) hFC analysis of platelet-free plasma spiked with 1 × 10⁸ U87 derived PpIX-positive EVs. (E) Linear regression plot of the % of particles in the PpIX gate versus the number of PpIX EVs spiked in. Goodness of fit, R²: 0.9998. (F) hFC analysis of PpIX particles in platelet-free plasma from control (10 min measurement). (G) hFC analysis of PpIX particles in platelet-free plasma from a patient treated with 5-ALA (10 min measurement). (H) Number of particles in the PpIX gate per patient group detected during 5 min of measurement.