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. 2020 Nov 7;12(11):3297. doi: 10.3390/cancers12113297

Figure 3.

Figure 3

ddPCR analysis of PpIX-positive EVs. (A) Number of copies of miR-21 found in the indicated number of sorted PpIX-positive EVs from U87 cells treated with 5-ALA. EVs isolated by ultracentrifugation were diluted in PBS and sorted with high-resolution flow cytometry. NTC: non-template control. EVs: extracellular vesicles. (B) Number of copies of miR-21 found in the indicated number of sorted PpIX-positive EVs from U87 cells treated with 5-ALA. EVs were isolated by ultracentrifugation, diluted in healthy donor plasma, and sorted with high-resolution flow cytometry. (C) Number of copies of miR-21 in PpIX-positive EVs sorted from a patient with GB after receiving 5-ALA. (D) Number of copies of miR-10b in PpIX-positive EVs sorted from a patient with GB after receiving 5-ALA. (E) Number of copies of Let-7a in PpIX-positive EVs sorted from a patient with GB after receiving 5-ALA.