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. 2020 Nov 9;9(11):1521. doi: 10.3390/plants9111521

Table 2.

Callus induction in L. glutinosa from stem cultured on MS medium treated with various concentrations of auxins (2,4-D and IAA) and 2 cytokinin (kinetin (KIN) and BA) hormones after four weeks of cultured.

Hormone Concentration (mg/L) % Callus Induction Fresh Weight
(g)
Dry Weight
(g)
TSC
2,4-D 0.5 70 ± 14.14 c 0.53 ± 0.24 b 0.03 ± 0.01 e 1.91 ± 0.03 de
1.0 76 ± 16.97 bc 0.62 ± 0.25 b 0.09 ± 0.05 b 2.01 ± 0.04 d
2.0 48 ± 5.66 de 0.61 ± 0.13 b 0.13 ± 0.03 a 2.00 ± 0.16 d
IAA 0.5 90 ± 8.49 ab 0.84 ± 0.47 a 0.07 ± 0.03 c 2.56 ± 0.01 c
1.0 92 ± 5.66 ab 0.56 ± 0.36 b 0.07 ± 0.05 c 2.73 ± 0.07 b
2.0 100 ± 0.00 a 0.62 ± 0.29 b 0.05 ± 0.03 d 3.12 ± 0.10 a
KIN 0.5 34 ± 8.49 ef - - -
1.0 32 ± 5.66 ef - - -
2.0 20 ± 5.66 f - - -
BA 0.5 64 ± 5.66 cd 0.06 ± 0.03 c 0.01 ± 0.00 f 1.85 ± 0.01 e
1.0 66 ± 2.83 cd 0.06 ± 0.03 c 0.01 ± 0.00 f 1.79 ± 0.08 e
2.0 38 ± 2.83 ef 0.05 ± 0.01 c 0.01 ± 0.00 f 2.68 ± 0.04 bc

Data are expressed as the mean ± SD; means with the superscription letters (a–f) are significantly different at p ≤ 0.05.