Figure 3.

The pancreatic transcriptional program in MEFs treated with insulinoma-derived exosomes. (A) Bright-field microscopic images of the medium control and only MIN6-derived exosome-treated MEFs. The insulinoma exosomes (MIN6-derived) did not induce unregulated cellular proliferation in the treated MEFs. Scale bar = 100 µm. (B) Relative transcript levels of Pdx1 and Fsp1 in MEFs treated with only small molecules, only exosomes, or the exosome + small molecule combination, compared with the medium control cells in stage 1 of differentiation. * p < 0.05, ** p < 0.01, and *** p < 0.001. Statistical significance was determined by paired, two-tailed t-testing. The data shown represent the mean ± SEM of three independent experiments. ns = non-significant. Stage 1 medium consisted of basal medium + stage 1 small molecules. The medium control cells represent cells grown in basal medium.