Figure 4.

Nicotinamide reduced AngII-induced AAA formation. AngII was infused in LDLR KO mice via osmotic mini-pump, and mice were treated without or with nicotinamide in the drinking water. (A) AAA diameter (n = 3–10). (B) Representative histology; H&E, Mac-3 (macrophages), VVG (elastin fragmentation), MPO, and MCP-1. (C) Elastin break count (n = 6). (D) Representative zymogram (upper panel) and quantified data (bottom panel) for aortic MMP-2 and MMP-9 activities (n = 3). Data were analysed using one-way ANOVA followed by Bonferroni post hoc analysis. AngII, angiotensin II; H&E, haematoxylin & eosin; L, lumen; Mac, macrophage; MMP, matrix metalloproteinase; Nam, nicotinamide; VVG, Verhoeff-van Gieson.