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. 2020 Nov 6;12(21):21220–21235. doi: 10.18632/aging.103827

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Copyright: © 2020 He et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Effects of TGF-β1 on high phosphate-induced calcification markers in VSMCs cells. (A) Real-time PCR assay results show the effect of high phosphate and/or LY364947 on mRNA of Runx2 in VSMCs cells ("*" p<0.05 vs. control; "#" p<0.05 and "##” p<0.01 vs. high phosphate group). (B) Real-time PCR assay results show the effect of high phosphate and/or LY364947 on mRNA expression of SM22α in VSMCs cells ("*" p<0.05 and "**" p<0.01 vs. control; "#" p<0.05 vs. high phosphate group). (C) Western blot assay results show the effect of high phosphate and/or LY364947 on Runx2 and α-SMA in VSMCs cells; GAPDH was used as a loading control. (D) Quantification results of Western blot assay show the effect of high phosphate and/or LY364947 on Runx2 in VSMCs cells ("**" p<0.01 vs. control; "#" p<0.05 and "##" p<0.01 vs. high phosphate group). (E) Quantification results of Western blot assay show the effect of high phosphate and/or LY364947 on α-SMA in VSMCs cells ("**" p<0.01 vs. control; "#" p<0.05 and "##" p<0.01 vs. high phosphate group). (F) Western blot assay results show the effect of high phosphate and/or LY364947 on OPN in VSMCs cells; GAPDH was used as a loading control. (G) Quantification results of Western blot assay show the effect of high phosphate and/or LY364947 on OPN in VSMCs cells ("**" p<0.01 vs. control; "#" p<0.05 vs. high phosphate group). (H) Quantitative analysis results of Alizarin Red S staining show the effect of TGF-β1 on mineralization in VSMCs ("**" p<0.01 vs. control; "##" p<0.01 vs. high phosphate group). Pi: phosphate, LY364947: TGF-β type I receptor (TGFβRI) specific inhibitor.