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. 2020 Nov 7;12(21):21290–21307. doi: 10.18632/aging.103863

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Copyright: © 2020 Yan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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L-dopa and SAM treatment shows opposing effects on the angiogenesis and cell proliferation. (A and B) After treatment with the indicated concentrations of L-dopa or SAM for 24 h, endothelial tube formation was assessed using light microscopy. The average number of microtubules in 3 random horizons was analyzed using ImageJ software. (C) Cells were treated with L-dopa for 24 h and were incubated for another 2 weeks before fixation, staining and colony quantification. Clonogenic assays were performed in triplicate. (D) CCK-8 assay was using to evaluate the effect of L-dopa on the cell viability. (E) Colony formation assays show the effect of SAM on primary HUVECs. The quantitative results shown of three independent experiments are the mean ± SD. (F) CCK-8 assay was used to evaluate the effect of SAM on the cell viability. The asterisk (* or **) indicates a significant (p < 0.05 or p < 0.01, respectively) compared with control groups..