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. 2020 Nov 27;9:e61050. doi: 10.7554/eLife.61050

Figure 3. Increased vascularisation in BNP-treated infarcted hearts.

(A) Representative immunostainings against CD31 protein (green) on hearts removed from saline-(MI) and BNP-treated infarcted mice (MI + BNP) 10 days after surgery. Nuclei stained in blue with DAPI. Scale bars: 100 μm. (B) CD31 staining intensity measured on at least 10 different pictures per heart and per area 3, 10 and 28 days after MI. Number of pixel in BNP-injected mice related to the numbers of saline-injected mice. (C) CD31+ cell number counted on heart sections of the different area of saline- and BNP-treated infarcted hearts. Cells counted on at least 10 different pictures per area and mouse. (B–C): Individual values are represented and the means ± SEM are represented in red. Statistical analysis was performed only for groups with n ≥ 6. # p<0.05 for different variance between groups, *p<0.05 using unpaired T tests with or without Welch’s corrections.

Figure 3.

Figure 3—figure supplement 1. BNP injection led to increased vascularisation in unmanipulated hearts.

Figure 3—figure supplement 1.

(A). Quantitative relative expression of mRNAs coding for CD31 (pecam1 gene), von Willbrand factor (vwf gene), Ve-cadherin (cdh5 gene), eNOS (nos3 gene), VEGF (vegfa gene), Stem Cell antigen 1 (Sca-1) (ly6a gene), Wilms’ tumour 1 (wt1 gene) and CD34 (cd34 gene). (B) Representative immunostainings against CD31 protein (green) of saline and BNP-treated unmanipulated hearts. Nuclei stained in blue with DAPI. CD31 staining intensity measured on at least 10 different pictures per heart and per area. The numbers of pixel obtained for saline-treated hearts related to the numbers obtained in BNP injected hearts. All the results are means ± SEM, # p<0.05 for different variance between groups, *p≤0.05 using unpaired T tests with or without Welch’s corrections.
Figure 3—figure supplement 2. Few cardiac endothelial cells in infarcted and remote zone 3 and 10 days after infarction express the CD45 protein.

Figure 3—figure supplement 2.

The presence of CD31+ CD45+ cells was assessed by immunostainings on adult hearts 3 and 10 days after MI. (A) Representative pictures of the stainings 10 days after MI. (B–C) The numbers of double positive cells were counted (B) on heart sections (0.15 mm2) of the different area of saline (MI) and BNP-treated infarcted hearts (MI + BNP) and related (C) to the total number of CD31+ cells. Cells were counted on at least 10 different pictures per area and mouse. n = 4–6 mice per group. All the results are means ± SEM.