Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | HEK 293FT | Thermo Fisher Scientific | # R70007 RRID:CVCL_6911 |
Human embryonic kidney |
| Cell line (Homo sapiens) | HDMEC | Promocell | C-12212 | Primary human dermal microvascular endothelium |
| Cell line (Mus musculus) | MS-1 | ATCC | #CRL-2279 RRID:CVCL_D134 |
Pancreas/islet of Langerhans; endothelium |
| Antibody | Goat polyclonal anti-PECAM-1 | R&D systems | AF3628 | (IF 1:400) |
| Antibody | Rabbit polyclonal anti-NG2 | Merck | AB5320 | (IF 1:200) |
| Antibody | Guinea pig polyclonal anti-Insulin | Dako | A0564 | (IF 1:1000) |
| Antibody | Rabbit polyclonal anti-BBS4 | Proteintech | 12766–1-AP | (WB 1:1000) |
| Antibody | Rabbit polyclonal anti-EphrinB2 | Thermo Fisher Scientific | BS-10659R | (WB 1:500) |
| Antibody | Rabbit monoclonal anti-phospho-VEGFR2 Y1175 | Cell Signaling Technology | 2478 | (WB 1:1000) |
| Antibody | Rabbit monoclonal anti-VEGFR2 | Cell Signaling Technology | 9698 | (IF 1:200) (WB 1:1000) |
| Antibody | Rabbit monoclonal anti-phospho-PLCγ1 Y783 | Cell Signaling Technology | 2821 | (WB 1:1000) |
| Antibody | Rabbit monoclonal anti-PLCγ1 | Cell Signaling Technology | 2822 | (WB 1:1000) |
| Antibody | Rabbit monoclonal anti-phospho-Akt Thr308/473 | Cell Signaling Technology | 4051 | (WB 1:1000) |
| Antibody | Rabbit monoclonal anti-pan Akt | Cell Signaling Technology | 4691 | (WB 1:1000) |
| Antibody | Rabbit monoclonal anti-phospho- p44/42 MAPK Thr202/Tyr204 | Cell Signaling Technology | 4370 | (WB 1:2000) |
| Antibody | Rabbit monoclonal anti- p44/42 MAPK | Cell Signaling Technology | 4695 | (WB 1:2000) |
| Antibody | Mouse monoclonal anti-α-tubulin | Sigma-Aldrich | T8203 | (WB 1:2000) |
| Antibody | Donkey monoclonal anti-Rabbit IgG, peroxidase-linked species-specific whole antibody Secondary Antibody | GE Healthcare | 10794347 | (WB 1:6000) |
| Antibody | Sheep monoclonal anti-mouse IgG, peroxidase-linked species-specific whole antibody Secondary Antibody | GE Healthcare | 10196124 | (WB 1:6000) |
| Peptide, recombinant protein | Recombinant Human VEGF 165 | Peprotech | 100–20 | 100 μg/ml in 1XPBS |
| Peptide, recombinant protein | Recombinant Human VEGF 165, Biotinylated Protein | R&D systems | BT293 | 100 μg/ml in 1XPBS |
| Commercial assay or kit | Mouse VEGF Quantikine ELISA Kit | R&D systems | MMV00 | |
| Commercial assay or kit | PowerUp SYBR Green Master Mix | Thermo Fisher Scientific | A25742 | |
| Commercial assay or kit | Dextran, Texas Red, 70,000 MW, Neutral | Thermo Fisher Scientific | D1830 | 5 mg/ml in 1X PBS |
| Commercial assay or kit | Dextran, Fluorescein, 40,000 MW, Anionic | Thermo Fisher Scientific | D1844 | 2.5 mg/ml in 1X PBS |
| Commercial assay or kit | Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye | Thermo Fisher Scientific | C10337 | |
| Chemical compound, drug | 2-NBDG (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)−2-Deoxyglucose) | Thermo Fisher Scientific | N13195 | 5 mg/ml in 1X PBS |
| Chemical compound, drug | Streptozotocin | Sigma-Aldrich | S0130-50MG | |
| Software, algorithm | MATLAB | MathWorks | RRID:SCR_001622 | R2016a |
| Software, algorithm | Volocity | PerkinElmer (Massachusetts, USA) | RRID:SCR_002668 | |
| Software, algorithm | Fiji-ImageJ | http://imagej.net/Fiji | RRID:SCR_002285 |