Appendix 1—key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Mice, male | C67/BL6N | Charles River laboratories | Catalog: #027 | |
| Mice, male | Prox1-eGFP KI | This paper | eGFP gene was inserted at the end of endogenous Prox1 gene to generate this indicator line. The sperms of the animals are cryopreserved in Charles River Laboratories. | |
| Peptide, recombinant protein | Bovine serum albumin | Sigma-Aldrich | A7030 | |
| Peptide, recombinant protein | human angiotensin II | Sigma-Aldrich | A9525 | |
| Peptide, recombinant protein | recombinant human VEGF-C (Cys156Ser) protein VEGFC | R and D systems | 752-VC | |
| Other | minipump | Alzet | Alzet 1002 or Alzet 2006 | |
| Sequence-based reagent | Prox1-eGFP genotyping forward primer | This paper | PCR primer | The primer sequence is 5’TCCAGGCAACAGTTCTACAG. It can be ordered from Integrated DNA Technologies using the sequence. |
| Sequenced-based reagent | Prox1-eGFP genotyping reverse primer | This paper | PCR primer | The primer sequence is 5’TGCACATCAGATTGTCTAAGG. It can be ordered from Integrated DNA Technologies using the sequence. |
| Commercial assay or kit | human VEGFC Quantikine ELISA kit | R and D systems | DVEC00 | |
| Commercial assay or kit | V-PLEX Human VEGFC kit | MSD | K151LTD-1 | |
| Other | Telemeters | Data Sciences International | PA-C10 or HD-X10 | |
| Other | Vevo2100 or Vevo3100 | VisualSonics Inc | ||
| Other | doppler flow velocity system | Indus Instruments | ||
| Other | AimStrip Hb Hemoglobin test system | Germaine Laboratories | ||
| Other | Trizol reagent | ThermoFisher | 15596026 | |
| Commercial assay or kit | RNeasy mini kits | Qiagen | 74104 | |
| Commercial assay or kit | TaqMan RNA to Ct 1-step kit | Thermofisher | 4392938 | |
| Commercial assay or kit | high capacity cDNA reverse transcription kit | Thermofisher | 4368814 |
|
| Other | TaqMan gene expression master mix | Thermofisher | 4369016 | |
| Other | Tapman probes for mouse | Thermofisher | Nppb, Mm01255770_g1; Pdpn, Mm01348912_g1; Lyve1, Mm00475056_m1; Flt4, Mm01292604_m1; Vegfc, Mm00437310_m1; Col1a1, Mm00801666; Col1a2, Mm00483888; Col3a1, Mm00802300_m1; Fn1, Mm01256744; Cd68, Mm03047343_m1; Cxcl10, Mm00445235_m1; Ptprc, Mm01293577_m1; Itgax, Mm00498701_m1; Itgam, Mm00434455_m1; Il1b, Mm00434228_m1; Il6, Mm00446190_m1; Ccl6, Mm01302419_m1; Ccl4, Mm00443111_m1; Ccl3, Mm00441259_g1; Ace2, Mm01159006_m1; Hprt, Mm03024075_m1 | |
| Other | QuantStudio 7 Flex Real-Time PCR System | ThermoFisher | ||
| Antibody | Anti-mouse Podoplanin antibody (Goat polyclonal) |
R and D systems | AF3244 | 1:500 dilution for Western blot |
| Antibody | anti-Akt antibody (Rabbit polyclonal) |
Cell signaling | #9272 | 1:1000 dilution for Western blot |
| Antibody | anti- p-Akt (Ser473) antibody (Rabbit polyclonal) |
Cell signaling | #9271 | 1:1000 dilution for Western blot |
| Antibody | p44/42 MAPK (Erk 1/2) antibody (Rabbit polyclonal) |
Cell signaling | #9102 | 1:2000 dilution for Western blot |
| Antibody | phsopho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Rabbit monoclonal) | Cell signaling | #4370 | 1:2000 dilution for Western blot |
| Antibody | GAPDH antibody (Rabbit monoclonal) | Cell signaling | #5174 | 1:5000 dilution for Western blot |
| Antibody | Lyve1 antibody (Rabbit polyclonal) | Abcam | ab14917 | 1:500 dilution for IF |
| Antibody | podoplanin antibody (Hamster monoclonal) | Abcam | ab11936 | 1:250 dilution for IF |
| Antibody | CD68 antibody (Rat monoclonal) | ThermoFisher | MA5-16674 | 1:300 dilution for IF |
| Antibody | smooth muscle actin antibody (Mouse monoclonal) | Abcam | ab7817 | 1:100 dilution for IF |
| Antibody | GFP antibody (Chicken polyclonal) | Abcam | ab13970 | 1:100 dilution for IF |
| Commercial assay or kit | trueview autofluorescence quenching kit | Vector laboratories | SP-8400–15 | |
| Other | Alexa 594-conjugated WGA | ThermoFisher | W11262 | 1:200 dilution for IF |
| Software, algorithm | Pathology image analysis software Visiopharm | Visiopharm | ||
| Other | FITC-Dextran | Sigma-Aldrich | FD500S | |
| Other | alexa Fluor 555 Cadaverine | ThermoFisher | A30677 | |
| Other | Qdot 655 | ThermoFisher | Q21021MP | |
| Other | Hamilton neuros micro-injection syringe | Hamilton | 6546006 | |
| Antibody | PE-Cy7 Mouse Anti-Mouse CD45.2 antibody (Mouse monoclonal) |
BD Pharmingen | 560696 | 1:100 dilution for FACS |
| Antibody | APC Rat Anti-Mouse CD31 Clone MEC 13.3 (RUO) antibody (Rat monoclonal) |
BD Pharmingen | 561814 | 1:100 dilution for FACS |
| Other | DAPI | Thermo Fisher | D1306 | 1:5000 dilution for FACS |
| Commercial assay or kit | RNeasy MinElute cleanup kit | Qiagen | 74204 | |
| Commercial assay or kit | Smart-Seq ultra low input RNA kit | Takara | 634890 | |
| Commercial assay or kit | Nextera DNA XT Library Prep Kit | Illumina | FC-131–1096 | |
| Commercial assay or kit | NextSeq 500/550 High Output Kit | Illumina | 20024907 | |
| Software, algorithm | R | R-project | v.3.5.3 and v.3.6.1 | |
| Commercial assay or kit | U-plex chemokine combo 1 (Ms) kit | MSD | K15321K-1 | |
| Other | Siemens clinical analyzer | ADVIA Chemistry XPT system | ||
| Commercial assay or kit | BCP albumin assay kit | Sigma-Aldrich | MAK125-1KT | |
| Other | Taqman probes for human | Thermo Fisher | LYVE1, Hs00272659_m1; PDPN, Hs00366766_m1; FLT4, Hs01047677_m1; VEGFC, Hs01099203_m1; HPRT1, Hs02800695_m1 | |
| Software, algorithm | Graphpad Prism | Graphpad |